Company Profile

Founded in 1998, RIGOL is an ISO9001:2000 Quality Management System and ISO14001:2004 Environmental Management System Certified company, an emerging T&M leader and a technology innovator in Chemical Analysis and Electronic Measurement. RIGOL's premium line of products includes HPLC and UV-Vis Spectrophotometers, and General Purpose, RF Communication Electronic Instruments, which help the engineers, researchers, educators to address their measurement challenges in confidence with better affordability than ever before

RIGOL is headquartered in Beijing, China, with the branch offices in Cleveland, OH, United States and Munich, Germany. The company's 400 employees and more than 150 distributors or representatives offer products and services in over 60 countries/regions on six continents. More information about RIGOL is available at hplc.rigol.com

company
rigol

L-3000 HPLC System

Excellent Performance
  • 9000PSI working pressure
  • 2.5AU Linearity range
  • 100Hz Sampling rate
  • Rapid analysis achievable
  • More efficient, less consume
Flexible Configuration
  • Modularized design
  • Multi-channel pumps
  • Alternative injection modes
  • Alternative injection modes
  • Custom combination
Professional Service
  • Instrument installation & various training courses
  • Application & regulatory compliance service
  • Remote diagnose & prompt response
  • Preventive maintenance & service plans
  • Proactive and comprehensive service
l-3000hplc

Your professional manufacturing supplier of Chromatography/Spectrum instruments,plus delicate service & application solution.

Selection Reference

selectionreference

Typical Configuration

isocratic

Isocratic System with Manual Injector

This isocratic system could deliver one type of mobile phase. By combing the best performance cost ratio, system stability and maintenance simplicity,this system is an ideal solution for routine QA/QC analysis and GPC/SEC application. It can also be simply upgraded into gradient system with autosampler to boost your productivity.

Quaternary System with Autosampler

Quaternary system possesses the advantage of great flexibility, and it can further be automatized by combing our autosampler. This combination could fulfil the complex requirements from pharmacy, environmental, food safety, petrochemical engineering, agricultural & farming fields. With the control from RIGOL Chromatography workstation, it realized full automatic analysis, report, system flush, shut down and then raised up the efficiency.

quaternary
binary

Binary System with Autosampler

With excellent gradient precision & optimized delay volume, this binary system plus autosampler is ideal for complex gradient application or be used in combination with mass spectrometry. With the convenience of autosampler and various detector, the user could easily get more data.

l-3200

Performance Highlights

High Pressure Limit

L-3200 serial high pressure pump could reach 9000psi (62Mpa) working pressure with the flow rate up to 10mL/min. This high performance extend the application field from normal HPLC to Fast HPLC. With the help of 100Hz detecting and high precision sampling, you can take advantage of small piratical size column and reduce solvent consumption.

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Precise Flow Rate & Gradient

L-3200 serial high pressure injection pump could monitor the pressure status by its digital signal processer and adjust the motor operation status based on working parameters and solvent type. This real time feedback mechanism plus the high performance proportional valve realized the steady flow output as well as precise.

Stable Pressure

L-3200 serial high pressure pump’s pressure pulsation could be controlled under 10psi with best qualitative repeatability based on it state of art patent design, precise engineering and strict quality control.

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  • Column: back pressure tube (1000PSI)
  • Mobile Phase A: 1% isopropanol aqueous solution
  • Mobile Phase B: 0.2% acetone/1% isopropanol aqueous solution
  • Flow rate: 1.0ml/min
  • Temperature: ambient
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  • Chromatographic column: Manufacturer A C18(250 mm*4.6mm* 5μm)
  • Mobile Phase: 0.05mol/L phosphate buffer(pH = 3.5)/MeOH/acetonitrile (50/35/15)
  • Flow rate: 1.0ml/min
  • Temperature: 40℃
  • Detection wavelength: 254nm
  • Injection volume: 20μL

Working Principle

L-3200 serial high pressure pump provides isocratic, binary and quaternary model base on request.

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Special Design

Integrated Design

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Built-in Seal Wash Function

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l-3320

Performance Highlights

High Precision

L-3320 autosampler delivers high injection precision of 0.3% RSD, thanks to its high precision machining parts and advanced control algorithm. It also employ a design of changeable sampling loop, which extend linearity range and meet the need of more applications.

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Low Cross Contamination

The special design of needle washing assembly could clean interior and outside of the sampling needle separately, combing the patented sampling needle design and extra precision inner and outer polishing technology, it could reduce the carryover of sample and avoid cross contamination.

Multi Injection Mode

L-3320 support three sampling mode including full loop, partial loop and micro pick-up injection for different requires. It could be easily choice in the method editor without hardware replacement.

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Working Principle

Three Sampling Mode

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Special Design

Build-in Needle Wash Assembly

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Front Access to Maintenance Parts

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l-3500

Performance Highlights

High Sampling Rate

As support by unique high speed signal processing technology, L-3500 UV-VIS detector realized 100 Hz sampling rate, which make the detection of narrow peak with width lower than 5s possible. This high sampling rate also helps to record the chromatography peak accurately and get the best resolution.

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Wilder Linearity Range

Adopt unique light path design, multi color optical filter and high quality grating, the detector realized much lower stray light, and extend the linearity range to 2.5AU.

Low Noise

L-3500 have a high performance SNR by the patent electric and structure design.

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Working Principle

Dual Lamp Design

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Easy Maintenance Flow Cell

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Application Notes

L-3500 shows great stability, especially in those applications with long run time and low detecting wavelength, this advantage can be perfectly showen by the 120min run of Chinese medicine Ginseng.

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L-3500 can run wavelength program, which means switching detection wavelength during acquisition. This feature realized the programmable detection of several analytes with different chromophore.

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l-3520

Performance Highlights

High Resolution Full Spectrum Scanning

L-3520 has a 1024 diode array sensor with pixel resolution of 0.6nm. Combine with high quality optical grating, it could record full high resolution spectrum while chromatogram recording.

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High Sensitivity

L-3520 realized ± 0.6×10 to the power of -5 AU noise level by its advanced optical and electric design, and gain much lower detection limit.

High Sampling Rate

RIGOL remains and advances the high sampling rate character of UV detector, and keep 100 Hz sampling rate with full spectrum scanning. Users can acquires high resolution spectrum, and also could extract chromatogram for quantitative analysis.

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Working Principle

Easy Calibration Function

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Easy Accessed Lamp

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Application Notes

The choromophore of the eight phenols in olive oil varies a lot, those analytes must be monitored at different wavelength and the retention time is similar, those realities makes it impossible to analysis it by UV-VIS detector. DAD possess the ability of multi-wavelength detection and full spectrum acquisition, perfect for qualitative and quantitative analysis.

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Chromatographic conditions

  • Chromatographic column: Manufacturer A
  • C18(100mm*4.6mm*2.6μm) Mobile phase: 0.2% aqueous phosphoric
  • acid/acetonitrile; gradient elution Flow rate: 1.0mL / min
  • Column temperature: 28 ℃
  • Injection volume: 5μL
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l-3535

Performance Highlights

Low-Temperature Evaporation SEDEX Automated Gain Adjustement (SAGA)

SAGA is an innovative gain control available when it is driver-controlled by software, SEDEX LC automatically adapts the gain setting to avoid any off-scale saturation of the detector.

Easy Mantainanence

A single and dedicated SEDEX LC HPLC nebulizer and an innovative cell design minimize band broadening. This nebulizer covers the flow rate range from 100µL/min to 2.5mL/min and can be readily and quickly mounted and dismounted.

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Application

Determination of 15 underivatized amino-acids via HPLC-ELSD

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Determination of gentamycin sulfate injection via HPLC-ELSD

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l-3560

Performance Highlights

Broad Application

Detection is based on a universal property of all analytes and does not require the presence of a chromophoric group, electroactive group, etc.

Stable Performance

Base line will be rapidly balanced by the inner temperature control system. And prevent the influence due to the outside temperature floatation.

Easy Operation

Preposition operation panel will help you set the instrument in serval steps, and no need to revise the parameter during the operation. This will reduce the learning cost and enhance the efficiency.

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Application

Analysis of Mannitol according to EP method

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Analysis of Sugar and Furfural

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l-3100

Performance Highlights

Ultra Experience Operating

L-3000 HPLC system equipped with L-3100 serial solvent organizer, which could remove the bubble influence effectively, and reduce the pressure fluctuation & base line noise.

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Pole Washing System

The washing system composed with solvent organizer and pump coordination, it could wash the pole and sealing ring salt crystallization and prolong the service life.

High Efficiency Degasser

L-3120 solvent organizer equipped with 2-Channel degasser which will remove the remaining gas dissolution in the mobile phrase, and make the pump operation more stabilized and reduce the base line noise.

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Bubbles Damages

  • It will lead to the big base line noise, and influence the base line till it could not be regularly works.
  • It might raise the problem with check valve, especially will cause big pressure floatation once you choose the pressure floatation control option.
  • Gradient mixing will generate the bubbles due to different characters of solvents. None degassing solvent provide the condition of bubbles generation.
  • Influence the detector operation, likes fluorescence quenching, reduce the sensitivity of FLD.
l-3400

Performance Highlights

Excellent Testing Results

L-3000 HPLC system work with L-3400 column oven, which could minimize the impact of environmental factors and then guarantee the superior results.

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Preheating Function

Via preheating treatment, mobile phrase will reach rated temperature before coming into the column, so avoid the unbalance temperature status in the column, then avoid the influence of temperature floatation also.

Precision Temperature Control

L-3400 utilized the semiconductor heating technology, and avoid the over shoot of temperature raising, then ensure the stable temperature raising and precision control.

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What is the semiconductor temperature control?

Utilized the Peltier reaction of semiconductor material, once the DC go through two different semiconductor material assembly couple, the couple will absorb and release the heat, so it could realize the cooling or heating target.The feature is, there is no any mobile parts and more reliable also.

Clarity Chromatography Workstation

clarity

Clarity workstation furthers the capability of L-3000 series HPLC system with Analysis solution research and development, instrument control, data acquisition and processing, report generation, and traceable record management with versatile extensibilities.

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Direct instrument control plus intuitive user interface give user the simplicity of operating the instrument from software.

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Simple method editing interface and powerful data processing with more than twenty integral functions.

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Various calibration procedures and customizable report editor.

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Specifications

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Application and Solutions

Pharmaceutical Analysis

Determine the content of soybean isoflavones in Soybean Isoflavone Soft Capsule by RIGOL L-3000 HPLC system with core-shell column

Soybean isoflavones are a class of secondary metabolites formed in the growth of soybean. They are extracted from plant, similar structure with female hormone, so soybean isoflavones also called phytoestrogen. Soybean isoflavones can improve skin condition and osteoporosis, relieve the symptoms of menopause syndrome.

  • Chromatographic column: Manufacturer A C18(50mm*4.6mm*2.6um)
  • Mobile phase: Phosphoric acid solution (pH=3.0)/Acetonitrile; gradient elution
  • Flow rate: 1.0mL/min
  • Column temperature: ambient
  • Detection wavelength: 260nm
  • Injection volume: 5µL
pharma

Environment Protection

Content determination of phenols compounds by HPLC

Phenols pollution in environment field means that phenols compounds pollute water. Waste water with phenols is one of the greatest dangerous, and most widely polluted industry waste water. In this paper, 9 phenols compounds were determined by using RIGOL L-3000 HPLC system. The results show that the method is accurate and sensitive.

  • Chromatographic column: RIGOL compass C18(250mm*4.6mm*5µm)
  • Mobile phase: Water/Acetonitrile; gradient elution
  • Flow rate: 1.0mL/min
  • Column temperature: 40℃
  • Detection wavelength: 223nm
  • Injection volume: 5µL
environ

Food Safty

Simultaneously determining the content of 8 kinds of organic acids and Vitamins in fruit by using HPLC-DAD

Organic acids and vitamins are very important dry matter in fruits. They usually decide the special taste of fruits and play an important role in food science and nutriology field. In this paper, the new method can simultaneously determine organic acids and vitamins in fruits by using HPLC-DAD.

  • Chromatographic column: RIGOL Compass C18(2) (250mm*4.6mm*5µm
  • Mobile phase: Water/Acetonitrile; gradient elution
  • Flow rate: 0.6mL/min
  • Column temperature: 30℃
  • Injection volume: 10µL
food

Agriculture

Simultaneously determining the content of 4 kinds of sulfa-drugs in fodder by using HPLC-DAD

Sulfa-drugs are a class of chemical medicine for disease prevention and treatment of bacterial infections. If take sulfa-drugs standard exceeding livestock products as food for a long time, it might lead to the damage of hematopoietic system, agranulocytosis and so on problems. So that the application limit of sulfonamides in livestock and poultry raise up and play an important role in food safety field. In this paper, an accurate and sensitive method was established to determine the content of 4 kinds of sulfa-drugs in feed by using HPLC-DAD.

  • Chromatographic column: RIGOL Compass C18(250mm*4.6mm*5µ m)
  • Mobile phase: Water/Acetonitrile (75/25)
  • Flow rate: 1.0mL/min
  • Column temperature: 40℃
  • Injection volume: 5µL
agriculture

Chemical

Simultaneously determining the content of 6 kinds of UV absorbers in cosmetics

UV absorbers in cosmetics can reduce or absorb the ultraviolet rays, protect skin. Excess ultraviolet radiation will irritate skin and cause skin sensibility. The application of UV absorbers is managed and controlled strictly. In this paper, a new method is developed to determining the content of 6 kinds of UV absorbers in cosmetics.

  • Chromatographic column: Manufacturer A C18(250mm*4.6mm*5µm)
  • Mobile phase: A: MeOH/Tetramethylene oxide (250/450); B: Water/Perchloric acid (300/0.1); gradient elution
  • Flow rate: 1.0mL/min
  • Column temperature: 30℃
  • Detection wavelength: 311nm
  • Injection volume: 10µL
chemical

Antibiotics

Determining the content of terramycin, doxycycline, tetracycline and auromycin by HPLC

In the livestock farming field, all kinds of antibiotics are used for treatment of livestock disease. Tetracycline antibiotics are widely adopt based on its antibacterial effect and cheap price. Tetracycline antibiotics mainly include terramycin, doxycycline, tetracycline and auromycin.

  • Chromatographic column: Manufacturer A C18(250mm*4.6mm*5µm)
  • Mobile phase: MeOH/Acetonitrile/0.01mol/L Citric acid solution (12/18/70)
  • Flow rate: 0.8mL/min
  • Column temperature: 30℃
  • Detection wavelength: 360nm
  • Injection volume: 50µL
antibiotic

Customer Service

01. Installation

RIGOL provide installation service with instrument working principle introduction, standard operation procedures, daily maintenance and application developing.

02. After Sale Service

RIGOL has a professional service team, and build up the maintenance record via the clients information system, we also arrange periodicity visiting service and preventive suggestion.

03. Regulation Compliance

RIGOL provide the compliance service for HPLC system, including the IO, OQ, PQ and so on.We can also provide the related training course to match up with the inspection reques.

customer-service

Professional services

04. Application Support

A cross-field application team covering pharmaceutical analysis, food safety, Bioscience and environment field is ready to support. They are focus on solving the application problem raised from client, and will provide the integrated solutions.

05. Multiple Training

RIGOL provide abundant online and offline standard training course. And could provide pertinence training course with client application and will enhance the skill of client.

06. Comprehensive Service

RIGOL provide 360 degree, proactive service support. Our technical team would like to provide you the technical and maintenance support with fast reaction and reasonable suggestion. Field service engineer team is ready at any time for emergency issues.

professional-service

Headquarter

  • RIGOL SCIENTIFIC, INC.
  • Address: No.156, Cai He Village, Sha He Town, Chang Ping District, Beijing
  • Postcode: 102206 P.R.China
  • Tel: +86-10-80706688
  • Fax: +86-10-80706688-668

EUROPE

  • Lindbergh str. 4
  • 82178 Puchheim
  • Germany
  • Email: info-europe@rigoltech.com

USA

  • 10200 SW Allen Blvd, Suite C
  • Beaverton, OR 97005, USA
  • Email: info@rigol.com

Japan

  • Tonematsu Bldg. 5F, 2-33-8 Nihonbashi-Ningyocho
  • Chuo-ku,
  • Chuo-ku,
  • Email: info-japan@rigol.com

India

  • L-143, Sector 10,Jeevan Bima Nagar Bangalore
  • 560 075India
  • Email:info-india@rigol.com

Service & Support Email Address: service.chem@rigol.com

Sales & Service (INDIA):
Alovie Technologies
Plot#609, 1st floor, Pragathi Nagar,
Opp. to JNTUC, Kukatpally,
Hyderabad, Telangana.
Email: info@alovie.com
Website: http://www.alovie.com/
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